Coding

Part:BBa_K3686010:Design

Designed by: JiXiao Wu   Group: iGEM20_SZ-SHD   (2020-10-25)


Insecticidal protein from Bacillus thuringiensis (BTH-3), complete CDs, codon-optimized


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 205
    Illegal NgoMIV site found at 220
    Illegal NgoMIV site found at 1567
    Illegal NgoMIV site found at 1828
    Illegal NgoMIV site found at 1990
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon-optimized for expression in Escherichia coli.


Source

Genome of Bacillus thuringiensis (BTH-3), full amino acid sequence submitted under GenBank accession no. EF486523.

References